HeLa cell HeLa 细胞 NICR429 cell NICR429 细胞 3111C0002000000057 Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） HeLa HeLa cell HeLa is the first aneuploid epithelioid cell line obtained from human tissue by continuous culture. It was established from cervical cancer tissue of 31-year-old black women by Gey GO et al in 1951. The original tissue sections were re-observed and diagnosed as adenocarcinoma by Jones et al. It is known that this cell line contains the HPV 18 sequence of human papillomavirus and needs to be operated in a level 2 biosafety station. This cytokeratin is keratin-positive, and the expression amount of p53 is low, but the expression level of pRB (retinoblastoma suppressor) is normal. HeLa-cell morphology-NIFDC Human Cervical Carcinoma Cells; HeLa MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR429 National Institute for Food and Drug Control P108 Passage at 1: 2 ~ 1: 6; and passage once in 2 to 3 days. Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=429 http://www.cellresource.cn/pdf/20080108100951.pdf 1:2~1:6传代；2~3天1次。 Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D21S11:27,28；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；PentaD:8,15；PentaE:7,17；TH01:7；TPOX:8,12；vWA:16,18 A类 HeLa 细胞 HeLa-细胞形态-NIFDC HeLa是第一个来自人体组织经连续培养获得的非整倍体上皮样细胞系，它由Gey GO等在1951年从31岁女性黑人的宫颈癌组织建立。经原始组织切片重新观察，Jones等将其诊断为腺癌。已知该细胞系含有人乳头状瘤病毒HPV18序列，需在2级生物安全防护台操作。该细胞角蛋白阳性，p53表达量较低，但表达正常水平的pRB（视网膜母细胞瘤抑制因子）。 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS P108 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样 中国食品药品检定研究院 人宫颈癌细胞；HeLa 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 贴壁生长