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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0003684">
        <rdfs:label xml:lang="en">HeLa cell</rdfs:label>
        <rdfs:label xml:lang="zh">HeLa 细胞</rdfs:label>
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    <Class rdf:about="http://purl.obolibrary.org/obo/CLO_0054286">
        <rdfs:label xml:lang="en">NICR590 cell</rdfs:label>
        <rdfs:label xml:lang="zh">NICR590 细胞</rdfs:label>
        <rdfs:subClassOf rdf:resource="http://purl.obolibrary.org/obo/CLO_0003684"/>
        <ns2:CLO_0051622 xml:lang="en">3131C0001000700019</ns2:CLO_0051622>
        <ns2:CLO_0051628 xml:lang="en">94-106</ns2:CLO_0051628>
        <ns2:CLO_0037192 xml:lang="en">Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D19S433:13,14；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；TH01:7；TPOX:8,12；vWA:16,18</ns2:CLO_0037192>
        <ns2:CLO_0051629 xml:lang="en">Base Medium+8%DMSO+20%FBS</ns2:CLO_0051629>
        <ns2:CLO_0051636 xml:lang="en">Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</ns2:CLO_0051636>
        <ns2:CLO_0051633 xml:lang="en">Class B</ns2:CLO_0051633>
        <ns2:CLO_0051637 xml:lang="en">Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn.</ns2:CLO_0051637>
        <ns2:IAO_0000118 xml:lang="en">HeLa</ns2:IAO_0000118>
        <ns2:IAO_0000118 xml:lang="en">HeLa cell</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="en">HeLa is the first aneuploid epithelioid cell line obtained from human tissue by continuous culture. It was established from cervical cancer tissue of 31-year-old black women by Gey GO et al in 1951. The original tissue sections were re-observed and diagnosed as adenocarcinoma by Jones et al. It is known that this cell line contains the HPV 18 sequence of human papillomavirus and needs to be operated in a level 2 biosafety station. This cytokeratin is keratin-positive, and the expression amount of p53 is low, but the expression level of pRB (retinoblastoma suppressor) is normal.</ns2:CLO_0051626>
        <ns2:CLO_0051635 xml:lang="en">Hela-cell morphology-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051623 xml:lang="en">Human Cervical Carcinoma Cells; HeLa</ns2:CLO_0051623>
        <ns2:CLO_0051635 xml:lang="en">LDH检测报告HeLa-Isoenzyme-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="en">MDH检测报告HeLa-Isoenzyme-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051627 xml:lang="en">MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS</ns2:CLO_0051627>
        <ns2:IAO_0000118 xml:lang="en">NICR590</ns2:IAO_0000118>
        <ns2:CLO_0051630 xml:lang="en">Negative</ns2:CLO_0051630>
        <ns2:CLO_0051638 xml:lang="en">Passage at 1: 2 ~ 1: 6; and passage once in 2 to 3 days.</ns2:CLO_0051638>
        <rdfs:comment xml:lang="en">The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains).</rdfs:comment>
        <ns2:CLO_0051625 xml:lang="en">adherent growth</ns2:CLO_0051625>
        <ns2:CLO_0051624 xml:lang="en">epithelioid</ns2:CLO_0051624>
        <rdfs:seeAlso xml:lang="en">http://www.cellresource.cn/fdetail.aspx?id=590</rdfs:seeAlso>
        <ns2:CLO_0051627 xml:lang="en">http://www.cellresource.cn/pdf/20080108100951.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051638 xml:lang="zh">1:2~1:6传代；2~3天1次。</ns2:CLO_0051638>
        <ns2:CLO_0051628 xml:lang="zh">94-106</ns2:CLO_0051628>
        <ns2:CLO_0037192 xml:lang="zh">Amelogenin:X；CSF1PO:9,10；D13S317:12,13.3；D16S539:9,10；D18S51:16；D19S433:13,14；D21S11:27,28；D2S1338:17；D3S1358:15,18；D5S818:11,12；D7S820:8,12；D8S1179:12,13；FGA:18,21；TH01:7；TPOX:8,12；vWA:16,18</ns2:CLO_0037192>
        <ns2:CLO_0051633 xml:lang="zh">B类</ns2:CLO_0051633>
        <ns2:IAO_0000118 xml:lang="zh">HeLa 细胞</ns2:IAO_0000118>
        <ns2:CLO_0051626 xml:lang="zh">HeLa是第一个来自人体组织经连续培养获得的非整倍体上皮样细胞系，它由Gey GO等在1951年从31岁女性黑人的宫颈癌组织建立。经原始组织切片重新观察，Jones等将其诊断为腺癌。已知该细胞系含有人乳头状瘤病毒HPV18序列，需在2级生物安全防护台操作。该细胞角蛋白阳性，p53表达量较低，但表达正常水平的pRB（视网膜母细胞瘤抑制因子）。</ns2:CLO_0051626>
        <ns2:CLO_0051635 xml:lang="zh">Hela-细胞形态-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="zh">LDH检测报告HeLa-同工酶-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051635 xml:lang="zh">MDH检测报告HeLa-同工酶-SHCRC</ns2:CLO_0051635>
        <ns2:CLO_0051627 xml:lang="zh">MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS</ns2:CLO_0051627>
        <ns2:CLO_0051627 xml:lang="zh">http://www.cellresource.cn/pdf/20080108100951.pdf</ns2:CLO_0051627>
        <ns2:CLO_0051624 xml:lang="zh">上皮样</ns2:CLO_0051624>
        <ns2:CLO_0051636 xml:lang="zh">中国科学院上海生命科学研究院细胞资源中心</ns2:CLO_0051636>
        <ns2:CLO_0051623 xml:lang="zh">人宫颈癌细胞；HeLa</ns2:CLO_0051623>
        <ns2:CLO_0051629 xml:lang="zh">基础培养基+8%DMSO+20%FBS</ns2:CLO_0051629>
        <rdfs:comment xml:lang="zh">本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。</rdfs:comment>
        <ns2:CLO_0051637 xml:lang="zh">联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn</ns2:CLO_0051637>
        <ns2:CLO_0051625 xml:lang="zh">贴壁生长</ns2:CLO_0051625>
        <ns2:CLO_0051630 xml:lang="zh">阴性</ns2:CLO_0051630>
        <foaf:depicted_by rdf:resource="http://www.cellresource.cn/pic/20090406162028.jpg"/>
        <foaf:depicted_by rdf:resource="http://www.cellresource.cn/pic/20090406164525.jpg"/>
        <foaf:depicted_by rdf:resource="http://www.cellresource.cn/pic/201663022261514.jpg"/>
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