produces Hep 3B cell Hep 3B 细胞 NICR1565 cell NICR1565 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000376 60～82 Amelogenin：X；CSF1PO：8；D13S317：12，14；D16S539：10；D18S51：20；D19S433：12.2，14；D21S11：30，31；D2S1338：21，25；D3S1358：15，OL；D5S818：13；D7S820：8，10；D8S1179：12；FGA：18；TH01：6，7；TPOX：9；vWA：17； Base Medium+8%DMSO+20%FBS C2 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Hep-3b cells were established in 1979 and originated from a seven-year-old black boy with liver cancer. The cells produce alpha-fetoprotein, hepatitis B surface antigen (HBsAg), albumin, macroglobulin, alpha-antitrypsin, transferrin, complement (C3), C3 activator, fibronectin, etc, with extensive research value. Hep-3b-Species Identification-CRC Hep3b Hep3b cell Hep3b-Cell Morphology-PUMC Human Hepatocellular Carcinoma Cells; Hep3b MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR1565 Passage at 1: 4 ~ 1: 6, and change fluid for 2 times a week Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=1565 http://www.cellresource.cn/pdf/20080108100951.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4～1:6传代，每周换液2次 60～82 Amelogenin：X；CSF1PO：8；D13S317：12，14；D16S539：10；D18S51：20；D19S433：12.2，14；D21S11：30，31；D2S1338：21，25；D3S1358：15，OL；D5S818：13；D7S820：8，10；D8S1179：12；FGA：18；TH01：6，7；TPOX：9；vWA：17； A类 C2 Hep-3b-物种鉴定-CRC Hep-3b细胞建系于1979年，源自一位患有肝癌的7岁黑人男童。该细胞产生甲胎蛋白（alpha-fetoprotein)、乙肝表面抗原（BHsAg) 、白蛋白、巨球蛋白、α- 抗胰蛋白酶（alphal-antitrypsin)、转铁蛋白、补体（C3)、C3活性载体，纤维原等，具有广泛的研究价值。 Hep3b 细胞 Hep3b-细胞形态-PUMC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国医学科学院基础医学研究所基础医学细胞中心 人肝癌细胞；Hep3b 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长 alpha-fetoprotein complement C3 alpha-1-antitrypsin