has_quality has participant L 929 cell L 929 细胞 NICR1296 cell NICR1296 细胞 - 3153C0001000000022 56-68 Base Medium+8%DMSO+20%FBS Class A Fluorescence Method（-） Jinhuan Wang, Kunming Cell Bank, Chinese Academy of Sciences，650223, Tel:0871-5195375, Fax：0871-5191823, E-mail:kcb@mail.kiz.ac.cn Kunming Cell Bank, Chinese Academy of Sciences L-929 L-929 cell L-929-cell morphology-KCB L929 L929 cell Mouse Connective Tissue Fibroblast Cell Line; L-929 [L929] NICR1296 PN3 Passage at 1: 3; and passage once in 3-4 days RPMI 1640 (w/o Hepes) 15%NBS The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The subclone of parent L is one of the earliest established continuous cultures and L cell line is derived from the subcutaneous reticular space and adipose tissue of 100-day-old C3H/An male mice. It is APRT+ and HPRT+. adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=1296 http://www.cellresource.cn/pdf/20080108101040.pdf - 1：3传代；3-4天一次 56-68 A类 L-929 细胞 L-929-细胞形态-KCB L929 细胞 PN3 RPMI 1640 (w/o Hepes) 15%NBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院昆明细胞库 亲本L的亚克隆，是最早建立的连续培养物之一，L细胞源自100天的雄性C3H/An小鼠的皮下网眼状空隙和脂肪组织。APRT+，HPRT+。 基础培养基+8%DMSO+20%FBS 小鼠结缔组织L细胞株929克隆；L-929 [L929] 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 王金焕，中国科学院昆明动物研究所，650223，Tel：0871-5195375，Fax：0871-5191823，E-mail：kcb@mail.kiz.ac.cn 荧光法（-） 贴壁生长 positive 阳性 adenine phosphoribosyltransferase hypoxanthine phosphoribosyltransferase