MCF7 cell MCF7 细胞 NICR13 cell NICR13 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000013 72~84 Amelogenin：X；CSF1PO：10；D13S317：11；D16S539：11，12；D18S51：14；D19S433：13，14；D21S11：30；D2S1338：21，23；D3S1358：16；D5S818：11，12；D7S820：8，9；D8S1179：10，14；FGA：23，24，25；TH01：6；TPOX：9，12；vWA：14，15； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Human Breast Cancer Cells; MCF7 MCF7 MCF7 cell MCF7-Species Identification-PUMC MCF7-cell morphology-PUMC NICR13 PMID: 8168088 Passage at 1: 3 ~ 1: 6; and passage for 2 to 3 times a week. The cells were isolated from the pleural effusion of a 69 -year-old white female patient with breast cancer. The cells retain the characteristics of several differentiated mammary epithelial cells, including: the ability to process estradiol through cytosolic estrogen receptors and form domas; the cells express WNT7B oncogene; TNF-α can inhibit the growth of MCF-7 cell line; Anti-estrogen treatment can regulate the secretion of insulin-like growth factor binding protein (IGFBP). Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=13 http://www.cellresource.cn/pdf/20080108100710.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3~1:6传代；每周2~3次。　 72~84 Amelogenin：X；CSF1PO：10；D13S317：11；D16S539：11，12；D18S51：14；D19S433：13，14；D21S11：30；D2S1338：21，23；D3S1358：16；D5S818：11，12；D7S820：8，9；D8S1179：10，14；FGA：23，24，25；TH01：6；TPOX：9，12；vWA：14，15； A类 C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS MCF7 细胞 MCF7-物种鉴定-PUMC MCF7-细胞形态-PUMC PMID: 8168088 http://www.cellresource.cn/pdf/20080108100710.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人乳腺癌细胞；MCF7 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞是从一名69岁的白人女性乳腺癌患者的胸腔积液中分离建立的。该细胞保留了多个分化乳腺上皮的特性，包括：能通过胞质雌激素受体加工雌二醇并能形成隆突结构（domes）；该细胞表达WNT7B癌基因；TNF-α可以抑制MCF-7细胞的生长；抗雌激素处理能调节细胞胰岛素样生长因子结合蛋白（IGFBP）的分泌。 贴壁生长