expresses MCF7 cell MCF7 细胞 NICR822 cell NICR822 细胞 3142C0001000000054 Base Medium+8%DMSO+20%FBS C148 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn Human Breast Cancer Cells; MCF7 MCF7 MCF7 cell MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR822 Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells were isolated from the pleural effusion of a 69 -year-old white female patient with breast cancer. The cells retain the characteristics of several differentiated mammary epithelial cells, including: the ability to process estradiol through cytosolic estrogen receptors and form domas; the cells express WNT7B oncogene; TNF-α can inhibit the growth of MCF-7 cell line; Anti-estrogen treatment can regulate the secretion of insulin-like growth factor binding protein (IGFBP). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=822 http://www.cellresource.cn/pdf/20080108100951.pdf 1:3传代,2-3天传一代 A类 C148 MCF7 细胞 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS STR鉴定 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 人乳腺癌细胞；MCF7 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 该细胞是从一名69岁的白人女性乳腺癌患者的胸腔积液中分离建立的。该细胞保留了多个分化乳腺上皮的特性，包括：能通过胞质雌激素受体加工雌二醇并能形成隆突结构（domes）；该细胞表达WNT7B癌基因；TNF-α可以抑制MCF-7细胞的生长；抗雌激素处理能调节细胞胰岛素样生长因子结合蛋白（IGFBP）的分泌。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性 WNT7B