NIH-3T3 cell NIH-3T3 细胞 NICR18 cell NICR18 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000018 53~76 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Mouse Embryonic Fibroblast; NIH/3T3 NICR18 NIH-3T3-Species Identification-PUMC NIH-3T3-cell morphology-PUMC NIH/3T3 NIH/3T3 cell NIH/3T3 is a highly contact-inhibited continuous passage cell line established from NIH Swiss mouse embryo culture. In order to cultivate substrains more suitable for transformation analysis in morphological characteristics, NIH/3T3 cell lines were further subcloned in more than 5 rounds. This cell line is highly sensitive to the formation of transformation foci of Sarcoma virus and the propagation of leukemia virus, and is very useful for DNA transformation and transfection research. Ectromelia virus test is negative. PMID: 8648675 Passage the cells When the cell growth to 80% or 80% of the confluence, and avoid the confluence; passage at 1: 3 ~ 1: 6, and passage for 2 times a week. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=18 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 53~76 A类 C5 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS NIH-3T3-物种鉴定-PUMC NIH-3T3-细胞形态-PUMC NIH/3T3 细胞 NIH/3T3是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞株对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用。鼠痘病毒阴性。 PMID: 8648675 http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；NIH/3T3 当细胞生长至80%或80%以下汇合时即可传代，切勿达到汇合；1:3~1:6传代，每周2次。 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长