NIH-3T3 cell NIH-3T3 细胞 NICR406 cell NICR406 细胞 3-5 � 10 (4) viable cells/cm2 3111C0002000000034 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% Mouse Embryonic Fibroblast; NIH/3T3 NICR406 NIH-3T3-cell morphology-NIFDC NIH/3T3 NIH/3T3 cell NIH/3T3 is a highly contact-inhibited continuous passage cell line established from NIH Swiss mouse embryo culture. In order to cultivate substrains more suitable for transformation analysis in morphological characteristics, NIH/3T3 cell lines were further subcloned in more than 5 rounds. This cell line is highly sensitive to the formation of transformation foci of Sarcoma virus and the propagation of leukemia virus, and is very useful for DNA transformation and transfection research. Ectromelia virus test is negative. National Institute for Food and Drug Control Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=406 http://www.cellresource.cn/pdf/20080108100710.pdf 3－5×10（4）活细胞/cm2 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 胎牛血清，10% NIH-3T3-细胞形态-NIFDC NIH/3T3 细胞 NIH/3T3是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞株对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用。鼠痘病毒阴性。 http://www.cellresource.cn/pdf/20080108100710.pdf 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 小鼠胚胎成纤维细胞；NIH/3T3 成纤维细胞样 贴壁生长