NIH-3T3 cell NIH-3T3 细胞 NICR494 cell NICR494 细胞 3131C0001000300006 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Mouse Embryonic Fibroblast; NIH/3T3 NICR494 NIH/3T3 NIH/3T3 cell NIH/3T3 is a highly contact-inhibited continuous cell line established from NIH Swiss mouse embryo cultures, as with the same established method of randomly mating 3T3 and inbred BALB/c 3T3 strains. In order to cultivate substrains more suitable for transformation analysis in morphological characteristics, the established NIH/3T3 cell lines were further subcloned in more than five rounds. This cell line is useful for DNA transformation and transfection studies. Detection showed that the cells were limb bone malformations virus ectromelia virus-negative. NIH3T3-cell morphology-SHCRC Negative P(126+5) The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=494 http://www.cellresource.cn/pdf/20080108100710.pdf A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% NIH/3T3 细胞 NIH3T3-细胞形态-SHCRC P(126+5) http://www.cellresource.cn/pdf/20080108100710.pdf 与原始的随机交配3T3和近交系BALB/c 3T3建株方法一样，NIH/3T3，是从NIH Swiss小鼠胚胎培养物中建立的高度接触抑制的连续细胞株。 为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了五轮以上亚克隆。 这株细胞对DNA转化及转染研究十分有用。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；NIH/3T3 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性