NIH-3T3 cell NIH-3T3 细胞 NICR798 cell NICR798 细胞 3142C0001000000030 Base Medium+8%DMSO+20%FBS C84 Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS Mouse Embryonic Fibroblast; NIH/3T3 NICR798 NIH/3T3 NIH/3T3 cell NIH/3T3 is a highly contact-inhibited continuous passage cell line established from NIH Swiss mouse embryo culture. In order to cultivate substrains more suitable for transformation analysis in morphological characteristics, NIH/3T3 cell lines were further subcloned in more than 5 rounds. This cell line is highly sensitive to the formation of transformation foci of Sarcoma virus and the propagation of leukemia virus, and is very useful for DNA transformation and transfection research. Ectromelia virus test is negative. Negative Passage at 1: 3, and passage once in 2-3 days. STR Identification The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=798 http://www.cellresource.cn/pdf/20080108100951.pdf 1:3传代,2-3天传一代 A类 C84 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NIH/3T3 细胞 NIH/3T3是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株，建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞株对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感，对DNA转化及转染研究十分有用。鼠痘病毒阴性。 STR鉴定 http://www.cellresource.cn/pdf/20080108100951.pdf 中国典型培养物保藏中心细胞库 基础培养基+8%DMSO+20%FBS 小鼠胚胎成纤维细胞；NIH/3T3 成纤维细胞样 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性