P3X63Ag8 cell P3X63Ag8 细胞 NICR735 cell NICR735 细胞 3131C0001000800009 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Mouse Myeloma Cells; P3X63-Ag8 NICR735 Negative P3X63-Ag8 P3X63-Ag8 cell PN5 Passage at 1: 2. The cells can be overgrown within 3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell line is derived from a P3K cell line (tissue culture strain derived from plasma cell MOPC-21). The cells are resistant to 0.1 mM 8-azaguanine but dead in HAT medium. It is reported that it is cholesterol-deficient to the lack of 3-ketosteroid reductase activity. Detection showed that the cells is limb bone malformations virus (ectromelia virus)-negative. http://www.cellresource.cn/fdetail.aspx?id=735 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast semi-adherent growth 1:2。3天内可长满。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% P3X63-Ag8 细胞 PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 半贴壁生长 基础培养基+8%DMSO+20%FBS 小鼠骨髓瘤细胞；P3X63-Ag8 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 这株细胞源自P3K细胞株（源自浆细胞MOPC-21的组织培养株)。 能抗0.1 mM 8-氮杂鸟嘌呤但在HAT培养基中死亡。 据报道它是由于缺失了3-酮类固醇还原酶活性的胆固醇营养缺陷型。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 阴性