has_quality has expression SW 480 cell SW 480 细胞 NICR654 cell NICR654 细胞 3131C0001000700086 Amelogenin:X；CSF1PO:13,14；D13S317:12；D16S539:13；D18S51:13；D19S433:13；D21S11:30,30.2；D2S1338:17,24；D3S1358:15；D5S818:13；D7S820:8；D8S1179:13；FGA:24；TH01:8；TPOX:11；vWA:16 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class B Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Human Colorectal Adenocarcinoma Cells; SW480 [SW 480; SW-480] IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS LDH检测报告SW480-Isoenzyme-SHCRC LDH检测报告SW480=5-Isoenzyme-SHCRC NICR654 Negative PN Passage at 1: 2 ~ 1: 8; and change fluid once in 1~2 days. SW 480 SW 480 cell SW-480 SW-480 cell SW-480-DNA-Fingerprinting-SHCRC SW480 SW480 cell SW480 was derived from an in situ rectal adenocarcinoma from a 51-year-old white male patient, while SW620 was derived from a lymph node metastasis from the same patient in one year later. It is CSAp-negative, colon antigen 3-negative and keratin-positive; G → A mutation of p53 gene at codon 273 leads to Arg → His substitution; C → T mutation of codon 309 leads to pro-ras substitution; expression of p53 protein increases in the cells; The expression of oncogene c-myc, K-ras, H-ras, N-ras, myb, sis and fos is positive; expression of Oncogene N-myc was not dedected; no matrilysin(a kind of metalloproteinases associated with tumor invasion) is expressed. The cells were reported to express GM-CSF. There is a mutation in codon 12 of ras protooncogene and it can be used as a positive control for detecting this mutation by PCR. The cells had been passaged to the 91st generation when summited to the ATCC by A. Leibovitz in November 1978. SW480-cell morphology-SHCRC The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=654 http://www.cellresource.cn/pdf/20080108100822.pdf 1:2~1:8传代；1~2天换液1次。 Amelogenin:X；CSF1PO:13,14；D13S317:12；D16S539:13；D18S51:13；D19S433:13；D21S11:30,30.2；D2S1338:17,24；D3S1358:15；D5S818:13；D7S820:8；D8S1179:13；FGA:24；TH01:8；TPOX:11；vWA:16 B类 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 10%FBS LDH检测报告SW480-同工酶-SHCRC LDH检测报告SW480=5-同工酶-SHCRC PN SW 480 细胞 SW-480 细胞 SW-480-DNA-指纹-SHCRC SW480 细胞 SW480-细胞形态-SHCRC SW480源自一位51岁白人男性患者的原位直肠腺癌，而SW620源自同一病人一年后的淋巴结转移灶。 该细胞CSAp和直肠抗原3阴性；角蛋白阳性；p53基因第273位密码子的G →A突变引起Arg→His替代，309位密码子的C→T突变导致Pro→Ser替代；细胞p53蛋白表达水平升高；癌基因c-myc、K-ras、H-ras、N-ras、myb、sis 和fos的表达呈阳性；未检测到癌基因N-myc的表达；不表达Matrilysin（一种与肿瘤侵袭相关的金属蛋白酶）。有报道称该细胞表达GM-CSF。ras http://www.cellresource.cn/pdf/20080108100822.pdf 上皮样 中国科学院上海生命科学研究院细胞资源中心 人结直肠腺癌细胞；SW480 [SW 480；SW-480] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性 positive 阳性 FOS MYB NRAS