has_quality has expression SW 480 cell SW 480 细胞 NICR1683 cell NICR1683 细胞 3131C0001000700172 Amelogenin:X；CSF1PO:13,14；D13S317:12；D16S539:13；D18S51:13；D19S433:13；D21S11:30,30.2；D2S1338:17,24；D3S1358:15；D5S818:13；D7S820:8；D8S1179:13；FGA:24；TH01:8；TPOX:11；vWA:16 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human Colorectal Adenocarcinoma Cells; SW480 [SW-480] LDH检测报告SW480-Isoenzyme-SHCRC LDH检测报告SW480=5-Isoenzyme-SHCRC NICR1683 Negative P(99+5) RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% SW-480 SW-480 cell SW-480-DNA-Fingerprinting-SHCRC SW480 SW480 cell SW480 is derived from orthotopic rectal adenocarcinoma. The cell line and SW620 ATCC CCL-227 originated from lymph node metastasis in the same patient after one year. It is CSAp-negative, colon antigen 3-negative and keratin-positive. It is keratin-positive in immunoperoxidase staining. G → A mutation of p53 gene at codon 273 leads to Arg → His substitution; C → T mutation of codon 309 leads to pro-ras substitution. The expression level of p53 protein increases. The expressions of oncogene c-myc, K-ras, H-ras, N-ras, myb, sis and fos is positive. The expression of oncogene N-myc has not been detected. The cells do not express cytolytic enzymes, a metalloprotein associated with tumor invasion. There is a mutation in codon 12 of ras protooncogene and it can be used as a positive control for detecting this mutation by PCR. The cells had been passaged to the 91st generation when summited to the ATCC by A. Leibovitz in November 1978. The cells were reported to express GM-CSF. There is a mutation in codon 12 of ras protooncogene and it can be used as a positive control for detecting this mutation by PCR. The cells had been passaged to the 91st generation when summited to the ATCC by A. Leibovitz in November 1978. SW480-cell morphology-SHCRC The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1683 http://www.cellresource.cn/pdf/20080108101040.pdf Amelogenin:X；CSF1PO:13,14；D13S317:12；D16S539:13；D18S51:13；D19S433:13；D21S11:30,30.2；D2S1338:17,24；D3S1358:15；D5S818:13；D7S820:8；D8S1179:13；FGA:24；TH01:8；TPOX:11；vWA:16 A类 LDH检测报告SW480-同工酶-SHCRC LDH检测报告SW480=5-同工酶-SHCRC P(99+5) RPMI 1640 (w/o Hepes) 优质胎牛血清，10% SW-480 细胞 SW-480-DNA-指纹-SHCRC SW480 细胞 SW480-细胞形态-SHCRC SW480源自原位直肠腺癌。 SW620 ATCC CCL-227源自同一病人一年后的淋巴结转移。 CSAp和直肠抗体3阴性。角蛋白免疫过氧化物酶染色阳性。 p53基因第273位密码子的G -> A突变引起Arg -> His替代，309位密码子的C -> T突变导致Pro -> Ser替代。 细胞p53蛋白表达水平提高。 癌基因c-myc, K-ras, H-ras, N-ras, myb, sis 和fos的表达呈阳性。 癌基因N-myc的表达未做检测。 不表达细胞溶解酶，一种与肿瘤入侵相关的金属蛋白 http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人结直肠腺癌细胞；SW480 [SW-480] 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性 positive 阳性 FOS MYB NRAS