SW 620 cell SW 620 细胞 NICR207 cell NICR207 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000207 48~51，XY Amelogenin：X；CSF1PO：13，14；D13S317：12；D16S539：9，13；D18S51：13；D19S433：13；D21S11：30，30.2；D2S1338：24；D3S1358：15，16；D5S818：13；D7S820：8，9；D8S1179：13；FGA：24；TH01：8；TPOX：11；vWA：16； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Colorectal Adenocarcinoma Cells; SW620 [SW 620; SW-620] L15: Leibovitz Medium 10%FBS NICR207 P42 Passage at 1: 2 ~ 1:10; change fluid once in 2~3 days. SW 620 SW 620 cell SW-620 SW-620 cell SW480 was derived from an in situ rectal adenocarcinoma from a 51-year-old white male patient, while SW620 was derived from a lymph node metastasis from the same patient in one year later, and was established by Leibovitz A. This cell line is mainly composed of single small spherical cells and bipolar cells without microvilli. It synthesized only a small amount of carcinoembryonic antigen (CEA, 0.15 ng/106 cells/10 days), of high tumorigenic in nude mice; it can synthesize TGFα and matrilysin; it is keratin-positive, CSAp-negative and colon antigen 3-negative; it express c-myc、K-ras、H-ras、N-ras、Myb、sis、fos, and the expression of N-myc is not dedected. G → A mutation of p53 gene at codon 273 leads to Arg → His substitution; C → T mutation of codon 309 leads to pro-ras substitution; expression of p53 protein increases in the cells; The expression of oncogene c-myc, K-ras, H-ras, N-ras, myb, sis and fos is positive; expression of Oncogene N-myc was not dedected; no matrilysin(a kind of metalloproteinases associated with tumor invasion) is expressed. The cells were reported to express GM-CSF. There is a mutation in codon 12 of ras protooncogene and it can be used as a positive control for detecting this mutation by PCR. The cells had been passaged to the 91st generation when summited to the ATCC by A. Leibovitz in November 1978. SW620 SW620 cell SW620-Cell Morphology-PUMC SW620-Species Identification-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=207 http://www.cellresource.cn/pdf/20080108100839.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2~1:10传代；2~3天换液1次。 48~51，XY Amelogenin：X；CSF1PO：13，14；D13S317：12；D16S539：9，13；D18S51：13；D19S433：13；D21S11：30，30.2；D2S1338：24；D3S1358：15，16；D5S818：13；D7S820：8，9；D8S1179：13；FGA：24；TH01：8；TPOX：11；vWA：16； A类 L15: Leibovitz Medium 10%FBS P42 SW 620 细胞 SW-620 细胞 SW480源自一位51岁白人男性患者的原位直肠腺癌，而SW620源自同一病人一年后的淋巴结转移灶，由Leibovitz A等建系。该细胞系主要由单个的小圆球细胞和无微绒毛的双极细胞组成。它仅合成少量癌胚抗原（CEA，0.15 ng/106 cells/10 days），且在裸鼠中有高度的致瘤性；可合成TGFα和matrilysin；角蛋白阳性，CSAp和结肠抗原3阴性；表达c-myc、K-ras、H-ras、N-ras、Myb、sis、fos，未检测到N-myc的表达。P53基因273位密码子存在G→A突 SW620 细胞 SW620-物种鉴定-PUMC SW620-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100839.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人结直肠腺癌细胞；SW620 [SW 620；SW-620] 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长