U-937 cell U-937 细胞 NICR59 cell NICR59 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000059 54~60 Amelogenin：X；CSF1PO：12；D13S317：10，12；D16S539：12；D18S51：13，14；D19S433：14，16；D21S11：27，29；D2S1338：17，20；D3S1358：16；D5S818：12；D7S820：9，11；D8S1179：12，13；FGA：22，25；TH01：6，9.3；TPOX：8，11；vWA：14，15； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Histiocytic Lymphoma Cells; U937 [U-937] Maintain the cell concentration between 1 � 105 ~ 2 � 106/ml; change fluid once according to the cell concentration of 3 to 4. NICR59 PMID: 1083890 RPMI 1640 (w/o Hepes) 10%FBS The cells were isolated from the chest water of a 37-year-old white male with malignant histiocytic lymphoma by Nilsson K Laboratory in 1974. Studies since 1979 have shown that the cells can differentiate into terminal monocytes under the induction of human mixed lymphocyte culture supernatant, phorbol ester, Vit D3, γ-IFN, TNF and retinoic acid. This cell does not synthesize immunoglobulin, and is EBV-negative, and can produce lysozyme, β-2-microglobulin, and TNF-α after PMA stimulation, and express C3R, and can be used as a transfected host, and express Fas, with sensitivity to TNF and anti-Fas antibody. U-937 U-937 cell U937 U937 cell U937-Species Identification-PUMC Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=59 http://www.cellresource.cn/pdf/20080108101040.pdf monocyte suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 54~60 Amelogenin：X；CSF1PO：12；D13S317：10，12；D16S539：12；D18S51：13，14；D19S433：14，16；D21S11：27，29；D2S1338：17，20；D3S1358：16；D5S818：12；D7S820：9，11；D8S1179：12，13；FGA：22，25；TH01：6，9.3；TPOX：8，11；vWA：14，15； A类 C5 PMID: 1083890 RPMI 1640 (w/o Hepes) 10%FBS U-937 细胞 U937 细胞 U937-物种鉴定-PUMC http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人组织细胞淋巴瘤细胞；U937 [U-937] 单核细胞 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 维持细胞浓度在1×105~2×106/ml；根据细胞浓度3~4换液1次。 该细胞是由Nilsson K实验室于1974年从一名37岁的患有恶性组织细胞性淋巴瘤的白人男性的胸水中分离建立的。1979年来的研究显示该细胞在人混合淋巴细胞培养物上清、佛波酯、Vit D3、γ-IFN、TNF和维A酸的诱导下可以向终末单核细胞分化。该细胞不合成免疫球蛋白，EBV阴性；可产生溶菌酶、β-2-微球蛋白，受PMA刺激后可产生TNF-α；表达C3R；可作转染宿主；表达Fas，对TNF和抗Fas的抗体敏感。