U-937 cell U-937 细胞 NICR725 cell NICR725 细胞 3131C0001000700159 Amelogenin:X；CSF1PO:12；D13S317:10,12；D16S539:12；D18S51:13,14；D19S433:14,16；D21S11:27,29；D2S1338:17,20；D3S1358:16；D5S818:12；D7S820:9,11；D8S1179:12,13；FGA:22,25；TH01:6,9.3；TPOX:8,11；vWA:14,15 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Human Histiocytic Lymphoma Cells; U937 [U-937] NICR725 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% The U-937 cell line was established in 1974 by Sundstrom and Nilsson and was obtained from the hydrothorax of patients with histiocytic lymphoma. The results showed that U-937 cells could be cultured with human mixed lymphocytes culture supernatant, in which phorbol ester, vitamin D3, interferon γ, tumor necrosis factor and retinoic acid to induce the differentiation of terminal monocytes. The immunoglobulin product and Epstein-Barr virus expression of the cells were negative. The cells express Fas antigen and are sensitive to TNF and anti-Fas antibodies. This cell line was collected from the United States. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). U-937 U-937 cell U-937-cell morphology-SHCRC U937 U937 cell http://www.cellresource.cn/fdetail.aspx?id=725 http://www.cellresource.cn/pdf/20080108101040.pdf monocyte suspension growth 1:2。3天内可长满。 Amelogenin:X；CSF1PO:12；D13S317:10,12；D16S539:12；D18S51:13,14；D19S433:14,16；D21S11:27,29；D2S1338:17,20；D3S1358:16；D5S818:12；D7S820:9,11；D8S1179:12,13；FGA:22,25；TH01:6,9.3；TPOX:8,11；vWA:14,15 A类 PN5 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% U-937 细胞 U-937-细胞形态-SHCRC U-937细胞系由Sundstrom和Nilsson在1974年建立，取材于患组织细胞淋巴瘤病人的胸水。 研究表明：U-937细胞能被人混合淋巴细胞培养上清，佛波脂、维生素D3、γ干扰素、肿瘤坏死因子和维甲酸诱导终末单核细胞分化。 该细胞免疫球蛋白产物和EB病毒表达为阴性。 该细胞表达Fas抗原且对TNF和抗-Fas抗体敏感。此细胞系从美国收集而来。 U937 细胞 http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 人组织细胞淋巴瘤细胞；U937 [U-937] 单核细胞 基础培养基+8%DMSO+20%FBS 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性