has_specified_output cell line 细胞系 establishing cell line 建立细胞系 PERSON:Matthew Brush Person: Matthew Brush 2013-4-20 MHB: For cases of initial establilshment of a line from a primary culture, successive passaging and/or selection processes can confer increasing degrees of genetic stability and compositional homogeneity as compared to the input primary culture. Historically, many texts consider the first passage as the clearest point to define the beginning of a line. However, in practice it is more often that case that more than one passage, and possibly additional selective techniques, may be required before a culture is deemed to have sufficient stability and homogeneity to be considered cell line. This is the view taken in OBI. Regardless, what is important is that some intentional, experimental step has been taken to establish a more homogenous and stable culture that can be characterized and progatated over time. Person: Matthew Brush establishing cell line 这个过程是通过将原代细胞培养物传代至相对遗传稳定性和组成均一性细胞培养物，或通过对现有细胞系的一些实验性修饰以产生具有新特征的新细胞系（例如永生化或一些其他稳定的遗传修饰，或选择某些定义的子集）。 PERSON:Matthew Brush a process whereby a new type of cell line is created, either through passaging of a primary cell culture to relative genetic stability and compositional homogeneity, or through some experimental modification of an existing cell line to produce a new line with novel characteristics (e.g. immortalization or some other stable genetic modification, or selection of some defined subset). 2013-4-20 MHB：对于从原代培养物初始建立株系的情况，与输入原代培养物相比，连续传代和/或选择过程可赋予更多的遗传稳定性和组成均一性。 从历史上看，许多理论认为第一代是界定一个细胞系起始的最明确的时间点。 然而，实际上更常见的情况是，在认为培养物具有足够的稳定性和同质性以被认为是细胞系之前，可能需要多于一次的传代以及可能的附加的选择性技术。 这是OBI所采取的观点。 无论如何，重要的是，已经采取了一些有意的尝试性的步骤来建立一个更加同质和稳定的培养，这种培养可以随着时间的推移而被表征和发展。 建立细胞系 establishing cell culture 建立细胞培养 metadata complete 完整的元数据