has_specified_input has_specified_output achieves_planned_objective cell cell type 细胞 细胞类型 culture medium 培养基 material processing 物质加工 material transformation objective 物质转化目标 cultured cell population 培养细胞群 establishing cell culture 建立细胞培养 这个过程是通过从一个新鲜来源分离和培养一种或多种细胞或者对现有的细胞培养物进行有意的实验性修饰以形成一种新的细胞培养物或细胞系（例如将原代培养物传代以产生二次培养物或细胞系，或对现有培养物或细胞系的永生化或稳定遗传修饰）。 PERSON:Matthew Brush A 'cell culture' as used here referes to a new lineage of cells in culture deriving from a single biological source.. New cultures are established through the initial isolation and culturing of cells from an organismal source, or through changes in an existing cell culture or line that result in a new culture with unique characteristics. This can occur through the passaging/selection of a primary culture into a secondary culture or line, or experimental modifications of an existing cell culture or line such as an immortalization process or other stable genetic modification. This class covers establishment of cultures of either multicellular organism cells or unicellular organisms. A 'cell culture' as used here referes to a new lineage of cells in culture deriving from a single biological source.. New cultures are established through the initial isolation and culturing of cells from an organismal source, or through changes in an existing cell culture or line that result in a new culture with unique characteristics. This can occur through the passaging/selection of a primary culture into a secondary culture or line, or experimental modifications of an existing cell culture or line such as an immortalization process or other stable genetic modification. This class covers establishment of cultures of either multicellular organism cells or unicellular organisms. PERSON:Matthew Brush PERSON:Matthew Brush PERSON:Matthew Brush a process through which a new type of cell culture or cell line is created, either through the isolation and culture of one or more cells from a fresh source, or the deliberate experimental modification of an existing cell culture (e.g passaging a primary culture to become a secondary culture or line, or the immortalization or stable genetic modification of an existing culture or line). establishing cell culture 建立细胞培养 metadata complete 完整的元数据