is about is_specified_output_of bait-prey hybrid interaction evidence yeast one-hybrid evidence yeast one-hybrid assay The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED). ECO:0000066 eco A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors. MI:0432 sequence-specific DNA binding yeast one-hybrid assay