realizes is about is_specified_output_of concretizes differential methylation hybridization evidence A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment. CpG island microarray evidence eco ECO:0000065 Color is indicative of methylation status - be it hypo- (pseudo-green), hyper- (pseudo-red), or equal methylation (pseudo-yellow). ECO:0000069 in vitro methylation assay evidence regulation of DNA methylation assay DNA methylation profiling by array design methylation-sensitive restriction enzyme sequencing assay