is about is_specified_output_of split-ubiquitin functional complementation evidence MI:0112 split-ubiquitin assay The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquitin (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough to reconstitute.TAIR:TED A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein. eco ECO:0000074 protein fragment functional complementation evidence protein domain specific binding split-ubiquitin assay