has part has part realizes is about is_specified_output_of concretizes amplification of intermethylated sites evidence AIMS A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell. eco ECO:0000086 The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events. in vitro methylation assay evidence DNA methylation assay epigenetic modification identification objective amplification of intermethylated sites assay study design