realizes is_specified_output_of concretizes reverse transcription polymerase chain reaction evidence ECO:0000109 ECO:0000108 reverse transcription polymerase chain reaction transcription evidence Using reverse transcription-PCR, we found that primers designed to span the intergenic regions between zur and the znuC homolog, as well as znuC and znuB homolog each generated a PCR product (Figure 2A). This indicated that these genes were transcribed on the same mRNA. Taken together, the results of the RT-PCR analyses and the reporter fusion assays indicate that expression of SMU.1882 is up regulated in the presence of CovR. transcript levels (e.g. RT-PCR) The RT-PCR assay indicated that the sycO, ypkA and yopJ genes (designated as pCD12, pCD13 and pCD14 in Y. pestis 91001 [19], respectively) were transcribed as a single primary RNA (Fig. 1), and thereby these three genes constituted a single operon in Y. pestis Microtus strain 201. CollecTF RT-PCR A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression. The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate. eco RNA detection assay evidence assay transcription profiling by RT-PCR design transcription profiling by RT-PCR assay