has part has part realizes is about is_specified_output_of concretizes transcript expression evidence subtractive hybridization evidence Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries. eco subtractive hybridization ECO:0000118 A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'. gene expression information content entity assay transcription profiling assay transcription profiling design suppression subtractive hybridization differential expression analysis objective