realizes is about is_specified_output_of concretizes immunoprecipitation evidence chromatin immunoprecipitation evidence Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). mchibucos ChIP evidence A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence. 2010-11-16T01:29:08Z ECO:0000226 eco MI:0402 sequence-specific DNA binding assay protein binding site identification design assay using chromatin immunoprecipitation