is_specified_output_of quantitative polymerase chain reaction evidence A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample. qPCR evidence real-time quantitative PCR ECO:0000231 eco quantitative real-time PCR evidence Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA. Although qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173). qPCR can be used to quantitatively determine levels of gene expression. 2010-11-16T05:57:20Z real time polymerase chain reaction evidence real-time PCR evidence qRT-PCR evidence CollecTF mchibucos Q-PCR evidence quantitative PCR evidence DNA detection assay evidence real time polymerase chain reaction assay