chromosome conformation-based evidence 4C evidence ECO:0000234 eco 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation. circularized 3C chromosome conformation capture on chip 2010-11-19T01:20:28Z A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray. circularized chromosome conformation capture evidence mchibucos