chromosome conformation-based evidence 3C-seq evidence A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing. 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation. eco circularized 3C sequencing 2010-11-30T03:22:25Z mchibucos 3C sequencing circularized 3C-seq chromosome conformation capture sequencing evidence ECO:0000239