RNA-sequencing evidence support of intron positions by RNA-sequencing alignment evidence RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data. ECO:0000342 eco Support of intron positions by RNA-seq alignment evidence mchibucos 2013-04-03T04:22:05Z A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.