experimental evidence polysome profiling evidence 2019-04-15T12:00:00Z OMP Once isolated, gel electrophoresis, immunoblot, and other protein profiling techniques can be applied to garner more information. eco jbmunro ECO:0001592 A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined.