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    <!-- http://purl.obolibrary.org/obo/ECO_0000049 -->

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        <rdfs:label>reporter gene assay evidence</rdfs:label>
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    <!-- http://purl.obolibrary.org/obo/ECO_0001802 -->

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        <rdfs:label>beta-galactosidase reporter gene assay evidence</rdfs:label>
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        <oboInOwl:id>ECO:0001802</oboInOwl:id>
        <ns2:IAO_0000115>A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.</ns2:IAO_0000115>
        <oboInOwl:hasExactSynonym>beta-galactosidase assay evidence</oboInOwl:hasExactSynonym>
        <oboInOwl:hasAlternativeId>ECO:0000297</oboInOwl:hasAlternativeId>
        <oboInOwl:created_by>swolfish</oboInOwl:created_by>
        <oboInOwl:hasOBONamespace>eco</oboInOwl:hasOBONamespace>
        <oboInOwl:hasRelatedSynonym>lacZ reporter gene assay</oboInOwl:hasRelatedSynonym>
        <oboInOwl:hasExactSynonym>LacZ transcript localization evidence</oboInOwl:hasExactSynonym>
        <oboInOwl:hasExactSynonym>beta-gal reporter gene assay</oboInOwl:hasExactSynonym>
        <ns2:IAO_0000234>CollecTF</ns2:IAO_0000234>
        <ns2:IAO_0000112>For each of the three genes, there was a significant increase of beta-galactosidase activity in Deltazur compared to WT when they grew in TMH with the addition of zinc. Thus, Zur repressed the promoter activities of znuC, znuA and ykgM.</ns2:IAO_0000112>
        <oboInOwl:creation_date>2015-10-29T15:57:10Z</oboInOwl:creation_date>
        <rdfs:comment>The assay is often performed using a plasmid borne construction on a lacZ strain.</rdfs:comment>
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