has part has part is_specified_output_of bait-prey hybrid interaction evidence glutathione S-transferase pull-down assay evidence GST pull-down assay swolfish eco 2015-11-04T09:05:56Z CollecTF ECO:0001817 A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions. The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing. assay gene knock in immunoprecipitation assay