nucleic acid binding evidence hydroxyl-radical footprinting evidence A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel. ECO:0001818 eco 2015-11-04T09:20:26Z swolfish CollecTF