has part has part realizes is about is_specified_output_of has participant concretizes affinity evidence DNA adenine methyltransferase identification evidence ECO:0001824 eco swolfish A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site. The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII. CollecTF DamID 2015-11-05T15:56:13Z protein localization methyltransferase complex assay material combination binding assay protein binding site identification design