is_specified_output_of has participant nuclease protection assay evidence S1 nuclease protection assay evidence eco snadendla 2015-09-28T17:16:56Z A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA. ECO:0005521 CollecTF RNA protection assay nuclease S1