is_specified_output_of has participant immunofluorescence evidence confocal microscopy evidence immunofluorescence confocal microscopy evidence eco This allows visualization of sub cellular distribution of biomolecules of interest. A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection. 2016-03-18T12:48:42Z The endogenous protein expression and localization for WalR and WalK was also checked using confocal immunofluorescence microscopy. WalR could be localized to the cytoplasm of B. anthracis but WalK could not be detected once again (data not shown). snadendla ECO:0005600 imaging assay confocal microscope antibody binding detection by fluorescence quenching