structure determination evidence immunodetection assay evidence selective antibody-based structural conformation evidence Here, we describe the generation, characterisation, and utility of a monoclonal antibody that selectively binds with high affinity to the asymmetric TNF trimer–small molecule complex. The antibody helps to define the molecular dynamics of the apo TNF trimer, reveals the mode of action and specificity of the small molecule inhibitors, acts as a chaperone in solving the human TNF–TNFR1 complex crystal structure, and facilitates the measurement of small molecule target occupancy in complex biological samples. A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule. DisProt:BalintMeszaros ECO:0006194 snadendla eco