protein binding evidence MHC:ligand binding based MHC restriction evidence eco A type of protein binding evidence that employs an assay measuring binding of an MHC molecule to a ligand to determine that T cells can recognize the ligand when presented by that MHC molecule. Determining that the T cell response to peptide ASCKGLIY is HLA-B*35:01 restricted because an assay measuring binding of a MHC:ligand complex was performed [PMID:8617300]. 2024-06-09T05:36:49Z MHC binding assay IEDB:RandiVita ECO:0008080 snadendla