Nucleic acid sequence analysis Genetic variation analysis Variant calling Mutation detection Exome variant detection Variant mapping Somatic variant calling is the detection of variations established in somatic cells and hence not inherited as a germ line variant. Variant detection Germ line variant calling Detect, identify and map mutations, such as single nucleotide polymorphisms, short indels and structural variants, in multiple DNA sequences. Typically the alignment and comparison of the fluorescent traces produced by DNA sequencing hardware, to study genomic alterations. Allele calling de novo mutation detection 1.1 Genome variant detection Somatic variant calling Methods often utilise a database of aligned reads.