realizes has_participant role_of deoxyribonucleic acid analyte role Helicos sequencing assay Helicos sequencing PMID: 18388294. Single-molecule DNA sequencing of a viral genome. Philippe Rocca-Serra url:https://en.wikipedia.org/wiki/Helicos_single_molecule_fluorescent_sequencing Helicos sequencing assay A DNA sequencing by synthesis assay that identifiies the sequnece of billions of DNA molecules immobilized to a surface using DNA polymerase and fluorescently labeled nucleotides added one at a time. The sequencing process does not require an amplification step and is typically able to produce reads of 25 base pair length. Helicos sequencing is a DNA sequencing which allows sequence identification of billions of DNA molecules immobilized to a surface by using DNA polymerase and fluorescently labeled nucleotides added one at a time. The sequencing process does not requires amplification step and is typically able to produce reads of 25 base pair length. true single molecule sequencing DNA assay sequencer assay by sequencer metadata complete