bearer_of has characteristic normal normal cell line CGR8 The germ-line competent cell line CGR8 was established from the inner cell mass of a 3.5 day male pre-implantation mouse embryo (Mus musculus, strain 129). These pluripotent cells retain the ability to participate in normal embryonic development. Differentiation of CGR8 cells is inhibited by the pleiotropic cytokine Differentiation Inhibiting Activity (DIA) which is identical to Leukaemia Inhibiting Factor (LIF). Addition of DIA/LIF is essential and allows culture of CGR8 without the use of feeder layers. Cells are small and tightly packed. http://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=07032901&collection=ecacc_gc