part_of part of part of has_quality has quality has quality derives_from embryonic stem cell Homo sapiens female blastocyst Homo sapiens cell line embryonic cell line ESC derived cell line ES-I3 The I3 stem cell line was derived from donated blastocysts by removal of the trophoblast layer and further culturing of the intact inner cell mass on mitotically inactivated mouse embryonic fibroblast (MEF) feeder cells. Following injection into SCID mice, the I3 line formed teratomas that contained tissues from all three embryonic germ layers. This line exhibits a normal XX karyotype. These cells were provided by Alex Meissner and maintained in mTeSR medium. http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSM537624