realizes role_of ribonucleic acid analyte role in situ sequencing FISSEQ fluorescent in situ sequencing To support FISSEQ being a child of 'in situ sequencing': this would mirror the layout for 'in-situ hybridization assay'. However, it is not entirely clear if all FISSEQ assays involve a padlock probe, but rolling-circle amplification is applicable genome-wide profiling of gene expression in situ in fixed cells and tissues, in which RNA is converted into cross-linked cDNA amplicons and sequenced manually on a confocal microscope fluorescent in situ RNA sequencing Dani Welter PMID:25675209