isolation of cell population single cell isolation fluorescence-activated cell sorting ERO:0000720 FACS NCIt:C17353 FAC sorting A flow cytometry assay that provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. The cells are suspended in a stream of fluid and forced individually through a vibrating nozzle, then exposed to a laser beam and the resulting fluorescence and scattered light is detected. Finally the cells are sorted by applying an electrical charge to droplets of the fluid and deflecting it to the left or right using charged electrodes. Dani Welter