has_participant assay by high throughput sequencer high throughput sequencer ligation-free chromosome conformation capture assay GAM Genome architecture mapping (GAM) is an assay designed to analyze 3D chromatin structure without relying on digestion and ligation. The procedure involves cryosectioning samples in random orientation, and then single nuclear profiles are isolated by laser microdissection. Each nuclear profile then undergoes high-throughput sequencing, and subsequent analysis can generate a matrix of inferred proximity between genomic regions. Dani Welter