Nuclease-based genetic perturbation LbCas12a Lachnospiraceae bacterium-derived CRISPR-associated nuclease protein. Similar to other Cas12a orthologs, LbCas12a produces staggered cuts in the DNA and utilizes a T-rich protospacer adjacent motif (PAM), unlike the G-rich PAM required by Cas9. LbCas12a has intrinsic RNase activity, allowing for the processing of its own crRNA, which enables multiplexed genome editing. LbCas12a has increased activity at temperatures below 37°C compared to other Cas12a orthologs.