Nuclease-based genetic perturbation FnCas12a Francisella novicida-derived CRISPR-associated nuclease protein. Similar to other Cas12a orthologs, FnCas12a produces staggered cuts in the DNA and utilizes a T-rich protospacer adjacent motif (PAM), unlike the G-rich PAM required by Cas9. FnCas12a has intrinsic RNase activity, allowing for the processing of its own crRNA, which enables multiplexed genome editing. FnCas12a uses shorter and more abundant PAM sequences compared to other Cas12a orthologs.