Nuclease-based genetic perturbation MbCas12a Moraxella bovoculi-derived CRISPR-associated nuclease protein. Similar to other Cas12a orthologs, MbCas12a produces staggered cuts in the DNA and utilizes a T-rich protospacer adjacent motif (PAM), unlike the G-rich PAM required by Cas9. MbCas12a has intrinsic RNase activity, allowing for the processing of its own crRNA, which enables multiplexed genome editing. In addition to the canonical TTTV PAM, MbCas12a equally well recognizes non-canonical C-containing PAMs, such as TCTA, TTCA, TCCA, CTTA, CCTA, and CCCA.