transient viral transduction method adeno-associated virus transduction 2026-03-26T11:30:00Z AI agent Transient transfection method involving the transgene introduction into the cells of interest using engineered adeno-associated virus (AAV) particles. AAV particles can transduce both replicating and non-dividing cells. Engineered AAV DNA does not integrate into the host genome, but rather, resides episomally in the host nucleus. In comparison to adenoviral vectors, AAVs have low immunogenicity and cytotoxicity. adeno-associated viral vector PMID:30710128 AAV vector A transient viral transduction method that uses adeno-associated virus (AAV) vectors to deliver nucleic acid cargo into target cells, where the vector DNA predominantly persists as episomal concatemers with minimal integration into the host genome. AAV transduction