transient viral transduction method herpes virus transduction 2026-03-26T11:30:00Z herpes simplex viral vector AI agent PMID:39533042 HSV vector HSV transduction A transient viral transduction method that uses herpesviral vectors, derived from herpesviruses such as herpes simplex virus (HSV), to deliver nucleic acid cargo into target cells, particularly neurons, without permanent integration into the host genome. Transient transfection method involving the transgene introduction into the cells of interest using engineered Herpes simplex virus (HSV) particles. HSV particles can transduce both replicating and non-dividing cells. Engineered HSV DNA does not integrate into the host genome, but rather, resides episomally in the host nucleus. HSV vectors have comparatively large transgene capacity (approx. 30kB) and have low immunogenicity.